Aracus Amino Acid Analyzer from Membrapure
The AAA system of the new generation ARACUS makes analysis of amino acids more modern, fast and simple. It uses the classic means of routine analysis in amino acids by post-column derivatisation with ninhydrin, also known as the “gold standard”.
A new procedure was developed which allows for the analysis of physiological and hydrolysate amino acids with the same buffer system and column.
The ARACUS amino acid analyser has a compact design consisting of three individual units: the main unit, the autosampler and the eluent rack EluBox. The software is very user friendly, self-explanatory and meets the latest GLP criteria.
THE AMINO ACID ANALYSER ARACUS BASED ON A MODULAR CONCEPT IS THE IDEAL INSTRUMENT FOR THE ANALYSIS OF AMINO ACIDS IN RESEARCH, QUALITY CONTROL AND IN THE CLINICAL LABORATORY.
ARACUS Amino Acid Analyzer from Membrapure – innovative technology combined with the classical method.
The amino acid analyzer ARACUS with its modular concept is the ideal instrument for the analysis of amino acids in research, quality control and in the clinical laboratory. It combines innovative technologies with the classical routine analysis of amino acids by the post- column derivatisation with ninhydrin. This produces results with the highest precision, reproducibility and accuracy.
The setup of the instrument allows the continuous analysis of up to 96 samples or 192 samples. The samples are stored in the cooled autosampler and the samples are injected without sample loss. Washing the injection valve and syringe pump guarantees zero cross-contamination.
As a third option the ARACUS instrument is also available with a manual injection system.
The eluent rack contains 6 glass bottles. The fluid volume of each bottle is actively monitored during operation. Eluents, ninhydrin and washing reagent are selectively chosen through buffer selection valves and degassed in micro vacuum chambers. The innovative technology of the pump allows the simultaneous operation of two fluid lines (eluent side and reagent side), which guarantees constant mixing.
The maintenance free photometers detect the separated amino acids at 570 nm and 440 nm and the signals registered by the aminoPeak software.
The concept of the ready-to-use eluent and reagent kits allows the reproducible analysis of the amino acid samples. Each kit is produced under standardized procedures, tested and certified before shipping it to the customer. Depending on the application, each kit contains the necessary amount for either 500 or 1000 analysis runs.
ARACUS is operated with the user friendly, self-explanatory software iControl. Optimised separation programs are correlated with each sample for the analysis. Correlation with new samples can be done whilst the instrument is analyzing a sample. The current status of important instrument parameters is shown as well as the fluid volumes of each eluent and reagent bottle. As soon as a minimal value is reached, different alarm levels inform the operator. All operation parameters and events of each analysis are documented in a log-File.
Data Acquisition and Handling
The acquisition and analysis software aminoPeak records simultaneously two analogue channels (570 nm, 440 nm). Chromatograms are shown on-line. Internal data bank allows fast peak identification. Quantitative calculations are performed with internal or external standards, dilution and/or multiplication factors. Several analysis runs can be compared using compiler programs. The result of each analysis is documented in an individual report.
The systems allows analysis of physiological and hydrolysate amino acids with the same buffer system and separation column. It operates with and without inert gas atmosphere.
- Compact design with three individual units:
- Main unit: a double piston pump, column heating system; temperature range 20°-100°C, reactor for ninhydrin reaction; temperature range 50°-150°C
- Micro Photometer, maintenance free; wavelength 570 nm and 440 nm, photo diodes as light source (life time up to 8 years)
- Autosampler 96 with space for 2 x 48 1,5 ml (standard) vials or 2 micro plates (96 format), cooling of racks to 4°C by Peltier elements
- Eluent Rack EluBox contains 6 bottles; each 500 ml volume, inert gas system supply (option), active degassing system
- Integration software aminoPeakTM 2002: This software facilitates data acquisition with up to four asynchronous, independent data channels at 100 Hz or less.
- It has many possibilities making manual or automatical data analysis more comfortable & efficient:
* quantitative measurement using external or internal standards
* time-independent integration parameters for each data-channel
* statistical analysis
* self-explaining graphical user interface with online-help
Analysis of free amino acids in biological and medical applications (serum, plasma, urine, culture media) or food.
Analysis of proteins, peptides, feedstuff or nutrition after hydrolysis.
SPECIFIC AMINO ACIDS
MSUD is a example of a specific application with a dedicated short run programm.